Within 2h isolation from the follicle 100 % of oocytes underwent germinal vesicle breakdown and both the nucleolus and the nuclear envelope disappeared . after culturing for 6 h , prometaphase occurred in 90 % oocytes 本研究旨在探索一种崭新的、化学试剂诱导去核卵母细胞为核受体的、无透明带的、手工体细胞核移植方法。
2.
Germinal vesicle ( gv ) transfer is a technique developed in recent years , which may be a valuable research procedure to study cell cycle regulation and characterize the cytoplasmic - nuclear interplay during maturation and fertilization of oocytes 生发泡( gv )移植是最近几年才建立起来的一种技术。通过gv移植可以研究细胞周期调控、成熟及受精时细胞核与细胞质之间的相互作用。
3.
Protein kinase a is camp - dependent kinase , one of the most important signal transduction pathways , plays a pivotal role in growth , differentiation , tumor occur , cell cycle control , etc . pka activators such as camp , 8 - br - camp or phosphodiesterase ( pde ) inhibitor isomethyl butyl xanthine ( imbx ) or purified pka catalytic subunit all can inhibit germinal vesicle breakdown ( gvbd ) and meiotic maturation in mouse oocytes , also in xenopus oocytes 蛋白激酶a ( proteinkinasea ， pka )是依赖于camp的丝苏氨酸蛋白激酶，是重要的信号传导途径之一，广泛参与许多生命过程，包括生长、分化、肿瘤发生、细胞凋亡、细胞周期调控等。以小鼠卵母细胞为实验对象，给予pka激动剂camp 、 8 - br - camp或磷酸二酯酶抑制剂imbx ( isomethylbutylxanthine )均可抑制小鼠卵母细胞的胚泡破裂及减数分裂成熟。
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